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Extracellular proteolytic inventory from various hyperthermophiles. Pyrococcus furiosus and P. abyssi were grown at 95 °C, and T. profundus, T. peptonophilis and Thermotoga maritima were grown at 80 °C. The cells were removed after 14 h of growth and the extracellular enzymes were precipitated by addition of ammonium sulfate to 80%. Two µg of total extracellular protein was loaded onto a 12% SDS-polyacrylamide gel containing gelatin.
