Table 1.
Protease inventory in Pyrococcus species, Sulfolobus solfataricus and Thermotoga maritima.
| Pyrococcus furiosus | Pyrococcus abyssi | Pyrococcus horikoshii | Solfolobus solfataricus | Thermatoga maritima | |||||
| Locus | Function | Locus | Function | Locus | Function | Locus | Function | Locus | Function |
| ATP-dependent proteases | ATP-dependent proteases | ATP-dependent proteases | ATP-dependent proteases | ATP-dependent proteases | |||||
| pf_481472 | ATP-dependent LA (Lon)1 | PAB1313 | ATP-dependent LA (Lon)1 | PH0452 | ATP-dependent LA (Lon)1 | SSO0278 | Proteasome beta subunit | TM1633 | ATP-dependent protease LA (Lon) |
| pf_119316 | ATP-dependent regulatory subunit | PAB2233 | ATP-dependent regulatory subunit | PH0201 | ATP-dependent regulatory subunit | SSO0738 | Proteasome alpha subunit | TM1869 | ATP-dependent protease LA, putative |
| pf_169930 | Proteasome beta subunit (PsmB-2) | PAB2199 | Proteasome beta subunit (PsmB-2) | PH0245 | Proteasome beta subunit (PsmB-2) | SSO0766 | Proteasome beta subunit | TM0198 | ATP-dependent Clp protease, ATPase (clpC-1) |
| pf_1318448 | Proteasome beta subunit (PsmB-1) | PAB1867 | Proteasome beta subunit (PsmB-1) | PH1402 | Proteasome beta subunit (PsmB-1) | SSO0271 | 26S proteosome regulatory subunit | TM1391 | ATP-dependent Clp protease, ATPase (clpC-3) |
| pf_1467425 | Proteasome alpha subunit | PAB0417 | Proteasome alpha subunit | PH1553 | Proteasome alpha subunit | TM0146 | ATP-dependent Clp protease, ATPase (clpX) | ||
| ATP-independent proteases | TM0873 | ATP-dependent Clp protease, ATPase (clpC-2) frameshift | |||||||
| ATP-independent proteases | ATP-independent proteases | ATP-independent proteases | SSO1886 | Thermopsin precursor, probable | TM0695 | ATP-dependent Clp protease, proteolytic subunit (clpP) | |||
| pf_301754 | Pyrolysin | PAB1252 | Pyrolysin-like | PH0310 | Pyrolysin-like | SSO2037 | Thermopsin precursor, probable | TM0521 | Heat-shock protein HslV (clpQ) proteolytic subunit |
| pf_251719 | Periplasmic serine protease, putative | PAB2437 | Periplasmic serine protease, putative | PH0282 | Periplasmic serine protease, putative | SSO2194 | Thermopsin precursor related protein | TM0522 | Heat-shock protein HslU (clpY) ATPase |
| pf_176949 | Metalloprotease | PAB2195 | Protease, putative | PH0256 | Protease, putative | SSO2551 | Serine protease, subtilase family, putative | TM0580 | Cell division protein FtsH |
| pf_401364 | Metalloprotease | PAB1063 | Metalloprotease | PH0351 | Metalloprotease | SSO1141 | Protease related protein | ||
| pf_472643 | Metalloprotease | PAB1319 | Metalloprotease | PH1569 | Hypothetical protease IV | SSO2045 | Protease | ATP-independent proteases | |
| pf_699579 | Subtisilin-like protease | PAB1960 | Hypothetical protease IV | PH1704 | Intracellular protease I (PfpI) | SSO2098 | Tricorn protease | TM0747 | Carboxy-terminal protease |
| pf_1479679 | Protease IV | PAB0311 | Intracellular protease I (PfpI) | PH0979 | Hydrogenase maturation protease (hyc I) | SSO1067 | Intracellular protease (PfpI) | TM0516 | Clostripain-related protein |
| pf_1553191 | Alkaline serine protease | PAB0576 | Hydrogenase maturation protease (hyc I) | PH0630 | Hypothetical neutral protease | SSO1759 | Protease, putative | TM0643 | Clostripain-related protein |
| pf_1757236 | Putative protease | PAB0555 | CAAX Prenyl protease | PH0585 | Hypothetical neutral protease | SSO2015 | Protease, putative | TM1589 | Clostripain-related protein |
| pf_1599202 | Intracellular protease I (PfpI) | SSO2612 | HtrA-like Serine protease (periplasmic) | TM1823 | FtsH protease activity modulator HflC (hflC) | ||||
| pf_637303 | Hydrogenase maturation protease (hyc I) | Peptidases | Peptidases | SSO0464 | Hypothetical protein, put. protease | TM1822 | FtsH protease activity modulator HflK (hflK) | ||
| pf_754933 | Hypothetical protein | PAB1320 | Carboxypeptidase I174 | PH0465 | Carboxypeptidase I | SSO0087 | Hypothetical protein, put. metalloprotease | TM0571 | Heat-shock serine protease, periplasmic (htrA) |
| pf_1136394 | Conserved hypothetical protein (bacteriocin homolog) | PAB1418 | Protein similar to acylaminoacyl peptidase | PH0594 | Protein similar to acylaminoacyl peptidase | TM1346 | Processing protease, putative | ||
| PAB1159 | O-sialoglycoprotein endopeptidase (gcp-1) | PH1987 | O-sialoglycoprotein endopeptidase (gcp-1) | Peptidases | TM0785 | Protease, bacteriocin | |||
| Peptidases | PAB1047 | O-sialoglycoprotein endopeptidase (gcp-2) | PH0445 | O-sialoglycoprotein endopeptidase (gcp-2) | SSO3115 | Tricorn Protease interacting F factor F1 | TM0145 | Secreted metalloendopeptidase Gcp, putative | |
| pf_471039 | Carboxypeptidase I | PAB1434 | Methionine aminopeptidase (MAP) | PH0628 | Methionine aminopeptidase (MAP) | SSO2154 | Tricorn Protease interacting F factor F2 | TM0409 | Conserved hypothetical, probable secreted proteinase |
| pf_334012 | Protein similar to acylaminoacyl peptidase | PAB2178 | Proline-dipeptidase related protein | PH1902 | Proline-dipeptidase related protein | SSO2675 | Tricorn Protease interacting F factor F3 | TM0890 | Conserved hypothetical, probable membrane metalloprotease |
| pf_181525 | O-sialoglycoprotein endopeptidase (gcp-1) | PAB2178 | X-Pro dipeptidase | PH1902 | Putative proline-dipeptidase | SSO0010 | X-pro aminopeptidase | TM1704 | Hypothetical protein (homology to protease maturation) |
| pf_488067 | O-sialoglycoprotein endopeptidase (gcp-2) | PAB0762 | Prolyl endopeptidase | PH1262 | Prolyl endopeptidase | SSO0363 | Prolidase | TM0916 | Periplasmic serine protease, putative |
| pf_561253 | Methionine aminopeptidase (MAP) | PAB2388 | Membrane dipeptidase | PH1026 | Membrane dipeptidase | SSO0433 | O-sialoglycoprotein endopeptidase | ||
| pf_707740 | Proline-dipeptidase related protein | PAB0045 | D-aminopeptidase | PH0078 | D-aminopeptidase | SSO0434 | O-sialoglycoprotein endopeptidase | Peptidases | |
| pf_743892 | Putative proline-dipeptidase | PAB1637 | XAA-Pro dipeptidase (proline dipeptidase) | PH1149 | XAA-Pro dipeptidase (proline dipeptidase) | SSO1355 | Thermostable carboxypeptidase (cpsA-1) | TM0963 | Oligoendopeptidase, putative |
| pf_803543 | Prolyl endopeptidase | PAB1419 | Pyroglutamyl-peptidase I | PH0596 | Pyroglutamyl-peptidase I | SSO1952 | Thermostable carboxypeptidase (cpsA-2) | TM0847 | Conserved hypothetical protein |
| pf_848157 | Membrane dipeptidase | PAB2375 | Aminopeptidase from M42 family | PH0519 | Aminopeptidase from M42 family | SSO3105 | Thermostable carboxypeptidase 1 | TM0445 | Conserved hypothetical protein |
| pf_1775248 | D-aminopeptidase | PAB0437 | Aminopeptidase from M42 family | PH1527 | Aminopeptidase from M42 family | SSO1465 | Pyrrolidone carboxylate peptidase (pcp) | TM1572 | Signal peptidase I, putative |
| pf_1898123 | Putative aminopeptidase | PAB2096 | Aminopeptidase from M42 family | PH1821 | Aminopeptidase from M42 family | SSO1607 | Pyrrolidone carboxylate peptidase, put. (pcp-like) | TM1696 | Type IV prepilin peptidase |
| pf_1902688 | Putative aminopeptidase | PAB0723 | Acetylornithine deacetylase (ArgE)/peptidase | PH1153 | Acetylornithine deacetylase (ArgE)/peptidase | SSO1864 | Microsomal dipeptidase | TM0129 | Carboxypeptidase G2, putative |
| pf_1906416 | Putative aminopeptidase | PAB0581 | Succinyl-diaminopimelate desuccinylase/peptidase | PH1288 | Succinyl-diaminopimelate desuccinylase/peptidase | SSO0098 | Methionine aminoppetidase 2 | TM0874 | Conserved hypothetical protein |
| pf_1262501 | XAA-Pro dipeptidase (proline dipeptidase) | PAB0966 | Signal sequence peptidase I (SEC11) | PH0590 | Signal sequence peptidase I (SEC11) | SSO2088 | Peptidase, putative | TM0463 | Lipoprotein signal peptidase |
| pf_1224347 | Pyroglutamyl-peptidase I | PAB0869 | Peptidase, putative | PH0737 | Peptidase, putative | SSO2181 | Peptidase related protein | TM0772 | Conserved hypothetical |
| pf_383398 | Endoglucanase/peptidase | PAB1781 | X-Pro aminopeptidase pepQ-3 | PH0974 | Hypothetical dipeptidase | TM1048 | Endoglucanase (peptidase) | ||
| pf_1444497 | Endoglucanase (celM)/peptidase | TM1049 | Endoglucanase (peptidase) | ||||||
| pf_1717470 | Endo-1,4-beta-glucanase/peptidase | TM1050 | Endoglucanase (peptidase) | ||||||
| pf_1131554 | Acetylornithine deacetylase (ArgE)/peptidase | TM0042 | Aminopeptidase P, putative | ||||||
| pf_1890964 | Succinyl-diaminopimelate desuccinylase/peptidase | TM0365 | Aminopeptidase, putative | ||||||
| pf_329163 | Signal sequence peptidase I (SEC11) | TM1713 | Proline dipeptidase, putative | ||||||
| pf_679507 | Hypothetical protein | TM1478 | Methionine aminopeptidase (map) | ||||||
1The Lon protease in Pyrococcus lacks the ATP-binding domain, but was placed in the ATP-dependent protease section because there is no experimental evidence aside from the sequence to suggest otherwise.