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. 2005 Jan 6;1(5):293–301. doi: 10.1155/2005/250757

Figure 4.

Figure 4.

Purification and pH and temperature dependencies of recombinant T. kodakaraensis malic enzyme (Tk-Mae). (A) SDS-PAGE of purified recombinant Tk-Mae. Lanes: 1 = soluble fraction after sonication; 2 = soluble fraction after heat treatment at 80 °C for 20 min; 3 = after anion exchange chromatography with Resource Q; and 4 = after gel filtration chromatography with Superdex 200 10/30HR. (B) Effect of pH on enzyme activity. Symbols: ◆ = 50 mM acetate-NaOH (pH 4.5–5.5); ■ = 50 mM MES-NaOH (pH 5.5–7.0); ▲ = 50 mM HEPES-NaOH (pH 7.0–8.0); and ● = 20 mM Tris-HCl (pH 8.5–9.0). (C) Effect of temperature on enzyme activity. (D) Thermostability of Tk-Mae. Incubation temperatures: ◆ = 99 °C; ■ = 95 °C; and ▲ = 90 °C.