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Restoration of activity by zinc ions in sn-glycerol-1-phosphate (Gro1PDH). The Gro1PDH was dialyzed against 50 mM EDTA (pH 8.0) for 24 h at 4 °C, and then against 50 mM Tris-HCl buffer/0.15 M NaCl (pH 8.0). Activity was measured after incubation in 50 mM Tris-HCl buffer/0.15 M NaCl (pH 8.0) containing ZnCl2 at 4 °C for 1 h. (a) Wild type (○), D144N (▽) and D144A (□). (b) Wild type (●), D191N (▼), H271A (▲) and H287A (■).
