Table 1.
Comparison of deduced amino acid sequences of Methanosarcina mazei nif genes with those of other methanogenic and nitrogen-fixing microorganisms. Percentage of identity was calculated using the GCG program (Deveraux et al. 1984).
Gene | M. mazei | M. barkeri | M. acetivorans | Methanococcus | Methanothermobacter | Clostridium | Clostridium | Klebsiella |
Gö1 | (nif2) | maripalidus | thermoautotrophicus | acetobutylicum | pasteurianum | pneumoniae | ||
nifH | 100% | 91% | 90% | 60% | 61% | 69% | 68% (nifH1) | 62% |
glnB′ | 100% | 88% | 90% | 56% | 55% | 49% | – | – |
glnB″ | 100% | 48% | 76% | 43% | 48% | 48% | – | – |
nifD | 100% | 87% | 88% | 39% | 39% | 52% | 52% | 40% |
nifK | 100% | 91% | 91% | 45% | 40% | 51% | 49% | 38% |
nifE | 100% | 83% | 87% | 45% | 45% | 49% | 53% | 34% |
nifN | 100% | 75% | 85% | 40% | 36% | 41% | –1 | 26% |
1Sequence not available in public databases.