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. 2002 Mar 5;1(2):87–93. doi: 10.1155/2002/313147

Figure 2.

Figure 2.

Titration of Sso7d with 1-anilinonaphthalene-8-sulfonic acid (ANS). Details are provided in the Materials and methods. (A) Fluorescence emission spectra of 0.1, 0.8, 1.5, 3, 5, 7, 10 and 12 µM ANS (solid lines, from bottom to top) in the presence of 2 µM Sso7d; the spectrum of ANS alone is also reported (broken line). (B) Fluorescence titration curve of Sso7d with ANS; the inset shows the double-reciprocal plot of fluorescence titration data.