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. 2002 Mar 5;1(2):87–93. doi: 10.1155/2002/313147

Figure 4.

Figure 4.

Interactions of Sso7d and the ANS–Sso7d complex with lysozyme aggregates. Left: profile of eluate from Superose-6 gel filtration columns loaded with 50 µg of lysozyme aggregates (prepared as described in Materials and methods) that had been incubated for 20 min at 50 °C with (a) 20 µg of Sso7d or (b) 20 µg of the ANS–Sso7d complex in 0.5 ml of 10 mM Tris-HCl pH 7.5. Columns were eluted with 10 mM Tris-HCl pH 7.5, 0.2 M NaCl at a flow rate of 12 ml h–1. Lysozyme activity was assayed as described in Materials and methods. Right: silver-stained SDS-PAGE gel. Lane 1: 5-µg sample from column (a). Lane 2: 5-µg sample from column (b).