Table 1.
Composition of acid-labile allyl ether-bonded hydrocarbons of PL-4. Allyl ether bonds of PL-4 were degraded with 5% HCl-methanol at 80 °C for 1 h or 100 °C for 3 h. The products were partitioned with a Bligh-Dyer solvent. The chloroform-soluble products were separated by TLC into the unchanged archaetidylcholine (AC), lysoarchaetidylcholine (lyso AC), lysoarchaetidic acid (lyso AA), archaetidic acid (AA), and small amounts of by-products. Phosphate contents of the water-soluble product and of each spot on the TLC plates were determined.
| Fraction | Allyl ether bonded hydrocarbon in PL-4 | Allyl ether bonded hydrocarbon (%)1 | Non-allyl ether-bonded hydrocarbon (%)2 | |
| No. | % | |||
| Water-soluble (a) | 2 | 77 | ||
| Lyso AC (b) | 1 | 5 | 83 | |
| Lyso AA (c) | 1 | 6 | ||
| Unchanged AC (d) | 0 | 9 | ||
| AA + unknown (e) | 0 | 2 | 17 | |