Figure 3.

(A) Northern blot analysis of the P. furiosus tyrB (AroAT I) and tyrC (AroAT II) transcripts. Pyrococcus furiosus was grown with the following compounds as the primary carbon and energy source: 10 mM cellobiose (C), 40 mM pyruvate (P), or 5 g l^–1 tryptone (T).Pyrococcus furiosus was also grown in a chemically-defined medium with cellobiose as carbon source in the presence (C+) and absence (C–) of the aromatic amino acids. (B) Identification of the transcriptional start site for the tyrB and tyrC by primer extension analysis; arrows indicate major and minor primer extension product. (C) Sequence of the tyrB and tyrC promoter regions and alignment to the tyrB and tyrC promoter regions from P. abyssi and P. horikoshii. The transcriptional start site as determined by primer extension analysis is designated by an arrow. The TATA and BRE sequences are boxed. The putative ribosome binding site ( S/D) is shown and the final ATG of each sequence is the first codon of each gene.