Table 2.

Substrate specificity of rAspAT from P. furiosus. The amino acids (20 mM) were assayed with 5 mM α-ketoglutarate as the amino acceptor, and the α-ketoacids (5 mM) were assayed with 20 mM aspartate as the amino donor.

Substrate	Relative activity (%)
Aspartate	100
Glutamate	30
Alanine	1.2
Phenylalanine	1.0
Tyrosine	0.6
Tryptophan	1.0
α-Ketoglutarate	100
Pyruvate	8
Phenylpyruvate	3
α-Ketoisovalerate	< 0.01
α-Keto-α-methylvalerate	< 0.01