Table 2.
Estimation of molecular mass by dynamic light scattering measurements with the wild type (wt) amidase and the Y41C mutant. Abbreviation: RH = hydrodynamic radius.
| pH 8.0 | pH 4.0 | pH 8.0 | pH 4.0 | |
| RH (nm)1 | RH (nm)1 | Estimated molecular mass (kDa)2 | Estimated molecular mass (kDa)2 | |
| Wt amidase | 4.2 | 7.5 | 95.4 | 385.7 |
| Y41C mutant | 4.4 | 4.5 | 106.0 | 111.0 |
1 Mean hydrodynamic radius derived from the measured translational diffusion coefficient using the Stokes-Einstein equation. 2 Molecular mass estimated from RH assuming that the particles are spherical and of standard density.