Figure 4.

Northern blotting analyses of RNA transcripts obtained from the cluster saci-4 of S. acidocaldarius. (A) Transcripts from one strand were detected in total RNA extracted from cells grown to exponential (expon.) or stationary (stat.) phase. (B) Transcripts were observed from the complementary DNA strand at stationary phase. The approximate estimated nucleotide lengths of the RNA products are given. The minimal detection limit, using 26-nt. probes, was estimated at 15–16 nucleotides. (C) Denotes the location of the primers “a” and “b,” and “L” indicates the location of the putative transcriptional leader within the flanking sequence.