Table 2.

Substrate specificity of α-glucan phosphorylase from Pyrococcus furiosus in the synthetic and phosphorolytic directions in the presence of glucose-1-phosphate (G-1-P) after 30 min. Percent conversions were calculated as the amount of sugar phosphate remaining in the reaction after incubation at a specified time, divided by the amount of substrate used in the reaction, and then multiplied by 100. The percent G-1-P produced was expressed as percent relative intensity (RI) which was calculated as: (RI of G-1-P from oligosaccharide)/(RI of G-1-P from maltoheptaose) × 100. Substrate concentration was 7 mg ml^–1 for glycogen and 7 mM for maltose through maltoheptaose.

Substrate	G-1-P consumed (Synthetic)	G-1-P produced (Phosphorolytic)
Maltose	100	0
Maltotriose	95	35
Maltotetraose	90	61
Maltopentaose	86	78
Maltohexaose	82	85
Maltoheptaose	80	100
Glycogen	75	64