Figure 5.

Effect of a Dominant Negative Form of MYB23.

(A) Structure of the MYB23 translational fusion constructs with the SRDX repressor domain.

(B) Root hair phenotype for the wild-type and PMYB23:MYB23-SRDX lines. Bar = 100 μm.

(C) Relative activity of the GL2 promoter in the root tips of wild-type and PMYB23:MYB23-SRDX lines. GUS activity in the root tips of the wild type or the PMYB23:MYB23-SRDX line harboring PGL2:GUS was quantitatively analyzed from 4-d-old seedlings. At least 20 seedlings were used for each. The error bar indicates sd from four biological replicates (each biological replicate consists of three technical replicates).

(D) Expression of transcriptional reporters in the developing root epidermis of the wild-type and PMYB23:MYB23-SRDX lines. Asterisks indicate the H position. Bar = 50 μm.

[See online article for color version of this figure.]