Figure 3.

Degradation of Peroxisomes during the Infection-Related Morphogenesis of C. orbiculare.

(A) Peroxisome degradation during the appressorium formation process. Conidia from the wild-type strain expressing GFP-SKL were incubated either on glass or on cucumber tissue (plant). Merged differential interference contrast and GFP fluorescence images are shown. a, appressorium; c, conidium. Bar = 10 μm.

(B) Transmission electron micrograph of a conidium. In a preincubated conidium, abundant peroxisomes (P) and mitochondria (M) were observed. Bar = 2 μm.

(C) Transmission electron micrographs of an appressorium. By contrast, the number of peroxisomes was markedly reduced in an appressorium. An arrow indicates a putative peroxisome inside a vacuole. Bar = 2 μm.

(D) Preferential degradation of peroxisomes in appressoria. The numbers of peroxisomes (P) and mitochondria (M) in preincubated conidia (0 h) and appressoria (24 h) were counted. Each mean and standard deviation was calculated from eight transmission electron microscopy (TEM) sections.

[See online article for color version of this figure.]