Figure 7.

PBD-Dependent Localization of Atg26 in the Putative Preautophagosome Structure.

(A) Constructs for the GFP-based localization analysis of Co Atg26.

(B) Introduction of GFP-Atg26 but not of GFP-Atg26 lacking PBD restored the pathogenicity of the atg26 strain. Each transformant was inoculated onto the right half of a cucumber cotyledon and incubated for 1 week. The atg26 mutant was inoculated onto the left half of each cotyledon.

(C) Intracellular localization of GFP-CoAtg26. Mycelial cells of each transformant were observed by confocal microscopy. Bar = 10 μm.

[See online article for color version of this figure.]