Figure 1.

Overexpression of GLK Genes Leads to Enhanced Transcript Levels of Nuclear Photosynthetic Genes.

RNA gel blot analysis showing transcript levels in wild-type, double mutant (glk1 glk2), and double mutant lines overexpressing either GLK1 (35S:GLK1) or GLK2 (35S:GLK2). Plants were grown for 28 d under 30 or 100 μmol quanta·m⁻²·s⁻¹ (low light and moderate light, respectively) at 21°C. All tissue samples were harvested within 20 min, starting at 3 h after subjective dawn. Ten micrograms of total RNA was loaded per lane. Blots were exposed to a phosphor-imager screen for quantification; images shown are subsequent exposures to autoradiography film. Values below each blot denote the approximate fold-change relative to the wild type grown under low light, standardized to 25S rRNA on the ethidium bromide–stained gel.