Figure 3.

Subcellular Localization of SCAMP2 with Styryl Fluorescent Dyes.

Distribution of endocytotic marker dyes FM4-64 and AM4-65 in BY-2 cells expressing SCAMP2-YFP.

(A) Cells were incubated with medium containing FM4-64 on ice for 10 min and then washed with cold medium. Samples were then incubated at room temperature for 5 to 10 min, 30 to 40 min, and 90 to 120 min before collecting fluorescent images.

(B) Cells were incubated with medium containing FM4-64 for 2 min at room temperature, washed with medium, and then incubated at room temperature. Samples were observed 5 to 10 min after washing.

(C) Distribution of AM4-65 in BY-2 cells expressing SCAMP2-YFP. The cells were incubated with medium containing AM4-65 for 2 min at room temperature, washed with medium, and then incubated at room temperature. The samples were observed 5 to 10 min after washing. Bars = 20 μm.