Figure 3.

Effects of Overexpression of TED6 and TED7 on SCW Formation.

Zinnia cells were induced to transdifferentiate into TEs after electroporation with the indicated constructs for overexpression under control of the CaMV 35S promoter. GUS was used as an overexpression control. Cell numbers with visible SCWs, which were differentiating and mature TEs, were counted at 96 h of culture. Each data point presents the mean of three independent cultures ± sd. Asterisks show pairs of samples that are significantly different by the two-sample t test (P < 0.05).

(A) Overexpression of the full-length and the C-terminal domain (Leu-27 to Ala-95) of TED6.

(B) Overexpression of the full-length and the C-terminal domain (Trp-109 to Gly-300) of TED7.

(C) Co-overexpression of TED6 and TED7. Cells had been electroporated with a mixture of both full-length constructs.