Figure 4.

Expression Pattern of TED6 and TED7 Genes in Arabidopsis Roots.

(A) to (D) Differentiating vessel element-specific expression of At TED6_Pro:GUS ([A] and [C]) and At TED7_Pro:GUS ([B] and [D]). Open arrowheads, protoxylem vessels at the outermost position of the vascular system; closed arrowheads, metaxylem vessels inside.

(E) to (M) Differentiating vessel element-specific expression of At TED6_Pro:At TED6-YFP and At TED7_Pro:At TED7-YFP in the protoxylem ([E] to [G], At TED7_Pro:At TED7-YFP) and metaxylem ([H] to [M], At TED6_Pro:At TED6-YFP). Both constructs gave similar YFP signals (see Supplemental Figures 3C and 3H online).

Bright-field ([E], [H], and [K]), fluorescent field ([F], [I], and [L]), and merged images ([G], [J], and [M]) are aligned from the left to the right. Protoxylem vessel elements have annular and spiral SCW thickenings, whereas metaxylem vessel elements usually have reticulate and pitted SCW thickenings. Bars in (A) for (A) to (D) and in (E) for (E) to (M) = 25 μm.