Figure 6.

Interaction between At TED6 and IRX3 Proteins on Copurification and Coimmunoprecipitation Assays.

(A) Copurification of IRX3 protein with At TED6 protein by Protein A–purified anti-At TED6 antibody-based affinity column purification. IgGs from preimmune and anti-At TED6 sera were cross-linked to Protein A-sepharose. The fractions bound to IgG-Protein A-sepharose were subjected to immunoblot analysis using Protein A–purified anti-At TED6, anti-aquaporin (anti-all PIP), and anti-IRX3 antibodies.

(B) Coimmunoprecipitation of At TED6 protein by the anti-IRX3 antibody. Normal sheep IgG was used as a control precipitant. Total crude extract and precipitated fractions from wild-type plants and At TED6 RNAi lines were subjected to immunoblot analysis.

Antibodies used are shown on the right. Numbers on the left indicate size markers (kD).