Figure 7.

Interaction between At TED6 and IRX3 Proteins on BiFC Assay in SCW-Forming Cells.

Full-length YFP fusion constructs and the mixture of nYFP and cYFP fusion constructs were biolistically introduced into leaves of transgenic Arabidopsis in which ectopic TE differentiation was induced by VND7 expression. Bar in (A) for (A) to (D) = 100 μm.

(A) Expression of 35S_Pro:At TED6-YFP (18 h after the induction).

(B) Expression of 35S_Pro:YFP-IRX3 (18 h after the induction).

(C) BiFC signal in the combination of 35S_Pro:At TED6-cYFP and 35S_Pro:nYFP-IRX3 (18 h after the induction).

(D) Ectopic TEs induced by VND7 expression in leaves (3 d after the induction).