Table 1.
Confirmation of IH-Specific Expression of Fungal Genes Using Real-Time-RT-PCR
| Ct Valuesa
|
Calculated Fold Changeb | ||
|---|---|---|---|
| Gene (Fold Change) | Mycelium | IH at 36 hpi | |
| Actin | 24.8 ± 1.3 | 23.3 ± 1.2 | – |
| AVR-Pita1 (3) | N/D | 29.6 ± 1.4 | – |
| MGG_04795.6 (100) | N/D | 25.3 ± 3.1 | – |
| MGG_09693.6 (84) | 35.9 ± 1.2 | 25.8 ± 3.3 | 388 |
| MGG_11610.6 (71) | 33.5 ± 2.5 | 23.9 ± 2.5 | 274 |
| MGG_06224.6 (64) | 38.4 ± 1.2 | 29.3 ± 0.6 | 194 |
a
Ct values represent the fractional number of cycles at which the amount of the amplified target reached a fixed threshold. High Ct values indicate low abundance of the specific transcript. The Ct values represent the mean of two biological and two technical replications. Standard deviations are shown. N/D, not detected.
b
Relative expression (RQ value) in IH defining expression in mycelium as 1X, calculated as 2−(ΔΔCT). Ct values were normalized to the fungal actin gene. A fold change was not calculated for AVR-Pita1 and MGG_04795.6 because their expression was not detected in the mycelial samples.