TABLE 1.

Kinetic parameters of PUFA substrates with 15-lipoxygenase

Substrates (5–100 μm) were incubated with 39.8 units/ml of 15-lipoxygenase, 4.52 × 10^-3% (v/v) Tween 20 in oxygen-saturated 0.05 m sodium borate buffer (pH 9.0), and products were quantified using absorbance at 236 nm. Curve fits were created using the Michaelis-Menten model.

Parameter	DPAn-3	DPAn-6	DHA
Vmax (μm/min)	672.9 ± 31.8	487.1 ± 13.9	481.9 ± 21.4
Km (μm)	36.2 ± 4.1	14.3 ± 1.5	20.4 ± 2.8
Vmax/Km (min-1)	18.6 ± 2.3	34.1 ± 3.7	23.6 ± 3.4
R2	0.93	0.90	0.85