TABLE 2.

Products formed upon incubation of DHA, DPAn-3, and DPAn-6 with soybean 15-lipoxygenase

DHA, DPAn-3, and DPAn-6 were incubated with soybean 15-lipoxygenase in 0.05 m sodium borate buffer, pH 9.0, at a concentration of 100 μm, at 4 °C for 30 min, and products were reduced to the corresponding hydroxy compounds using sodium borohydride. Reactions were analyzed and product structures were elucidated based on LC-MS/MS analysis of diagnostic fragments. Diagnostic fragments that helped in ascertaining positions of hydroxylation are shown in parentheses.