TABLE 3.

Products formed upon incubation of DHA, DPAn-3, and DPAn-6 with porcine 12-lipoxygenase

DHA, DPAn-3, and DPAn-6 were incubated with 12-lipoxygenase obtained from porcine leukocytes in 0.1 m Tris-HCl, pH 7.5, 5 mm EDTA, and 0.03% Tween 20, at a concentration of 100 μm, at 4 °C for 30 min, and products were reduced to the corresponding hydroxy compounds using sodium borohydride. Reactions were analyzed by LC-MS/MS, and product structures were elucidated based on analysis of diagnostic fragments. Diagnostic fragments that helped in ascertaining positions of hydroxylation are shown in parentheses.