TABLE 4.

Products formed upon incubation of DHA, DPAn-3, and DPAn-6 with potato 5-lipoxygenase

DHA, DPAn-3, and DPAn-6 were incubated with potato 5-lipoxygenase in 0.05 m Na-MES buffer, pH 6.3, 0.02% C12E10 at a concentration of 100 μm, at 4 °C for 30 min, and products reduced to the corresponding hydroxy compounds using sodium borohydride. Reactions were analyzed by LC-MS/MS, and product structures were elucidated based on analysis of diagnostic fragments. Diagnostic fragments that helped in ascertaining positions of hydroxylation are shown in parentheses.