Western blot analysis of Bax in mitochondrial fractions (A),
followed by band optical density analysis (B), from control EC
(Con) or EC exposed to acidosis without (Ac) or with
treatment with inhibitors of PKA: 3 μmol/liter H-89 or 30 μmol/liter
(Rp)-adenosine 3′,5′-cyclic
monophosphorothioate (Rp-cAMPS). Western blot data are
representative of four independent experiments with similar results. Values
are means ± S.E. (n = 4). *, p < 0.05
versus acidosis. r.u., relative units.