FIGURE 3.

Effect of a JNK inhibitor on palmitate-induced alterations in insulin-stimulated phosphorylation of Akt and GSK-3 in H4IIEC3 hepatocytes. A, H4IIEC3 cells were incubated in the presence or absence of palmitate (Pal) and the JNK inhibitor SP600125 (SP) for 16 h prior to stimulation with insulin (1 ng/ml, 15 min). Total cell lysates were resolved by SDS-PAGE, transferred to a PVDF membrane, and immunoblotted with the indicated antibodies. Detection was enhanced by chemiluminescence. Representative blots are shown. B, the values from densitometry of four (p-Akt or p-GSK-3) independent experiments were normalized to the level of total Akt or GSK-3 protein, respectively, and expressed as the mean -fold increase over control ± S.E. **, p < 0.01 versus palmitate treatment.