FIGURE 7.

Effects of antioxidants on palmitate-induced JNK activation in H4IIEC3 hepatocytes. A, H4IIEC3 cells were incubated in the presence or absence of palmitate (Pal) and antioxidants for 16 h. Total cell lysates were resolved by SDS-PAGE, transferred to a PVDF membrane, and immunoblotted with the indicated antibodies. Detection was by enhanced chemiluminescence. Representative blots are shown. B, the values from densitometry of four (NAC orα-tocopherol) independent experiments were normalized to the level of total JNK protein and expressed as the mean -fold increase over control ± S.E. **, p < 0.01 versus palmitate treatment alone. Toc, α-tocopherol.