FIGURE 9.
Involvement of mitochondrial fatty acid oxidation in palmitate-induced ROS production. A, H4IIEC3 cells were incubated in the presence or absence of palmitate (Pal) and the CPT-1 inhibitor etomoxir (Eto) for 8 h. Intracellular ROS production was quantified using the fluorescent probe H2DCFDA. The values are expressed as mean -fold increase over control ± S.E. (n = 4). B, H4IIEC3 cells were incubated in the presence or absence of palmitate (Pal) or oleate (Ole) for 16 h. Total RNA was extracted and subjected to reverse transcription. Using the cDNA as a template, the amounts of CPT-1 mRNA were detected by real time PCR. The values were normalized to the level of 18 S ribosomal RNA and expressed as mean -fold increase over control ± S.E. (n = 3). *, p < 0.05 versus control. **, p < 0.01 versus palmitate treatment alone.