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. 2009 May 29;284(22):14866–14874. doi: 10.1074/jbc.M807208200

FIGURE 3.

FIGURE 3.

Barx2 and the HBS potentiate activation of the SMA promoter by MyoD. A, sequence of the 300-bp proximal SMA promoter region used in SMA promoter-luciferase reporter constructs. B, luciferase assay of the SMA promoter construct transfected into primary limb bud cells from Barx2+/– and Barx2–/– mice shows that Barx2 is required for full promoter activity. C, co-transfection of the SMA promoter construct with Barx2:pcDNA3, MyoD:pcDNA3, or both MyoD:pcDNA3 and Barx2:pcDNA3 plasmids in C2C12 cells shows that Barx2 cooperates with MyoD to increase SMA promoter activity. Co-transfection of the ΔHBS-SMA promoter construct with the same effector plasmids shows that mutation of the HBS element prevents cooperative activation by Barx2 and MyoD. Promoter activity is in arbitrary units, and experiments were performed at least four times. All values are significant at p < 0.05.