FIGURE 2.

Flow cytometry analysis of PAC-1 binding to WT and mutant αIIbβ3-expressing cells. Washed CHO cells were incubated without (white bars) or with 1.1 μg of anti-LIBS2 antibody (gray bars) or 10 mmol/liter DTT (black bars) for 20 min at room temperature. FITC-labeled PAC-1 was added for 45 min at room temperature, and bound PAC-1 was assessed by flow cytometry. Data are the means ± S.E. WT, CHO-αIIb/β3 (n = 4); RW, CHO-αIIb(RW)/β3 (n = 4); LM, CHO-αIIb(LM)/β3 (n = 4); RWLM, CHO-αIIb(RWLM)/β3 (n = 4); SF, CHO-αIIb/β3(SF) (n = 3); RWLMSF, CHO-αIIb(RWLM)/β3(SF) (n = 3).