FIGURE 5.
Effects of LXRα and insulin on the LGK and FAS mRNA levels. Primary cultured hepatocytes were isolated from rat liver and infected with Ad-GFP (multiplicity of infection of 10) or Ad-DN-SREBP-1c (multiplicity of infection of 10) for 3 h. Cells were treated with insulin (100 nm) and/or T1317 (1 μm)/9-CR (1 μm) for 24 h in the absence of fetal bovine serum as indicated. mRNA levels of LGK (A) and FAS (B) were quantitated by real time PCR. The quantity of mRNAs was normalized with respect to β-actin mRNA. Data were processed by the comparative CT method and expressed as -fold increase relative to the basal transcription level in the absence of ligands.