TABLE 3.
Kinetic parameters of arsenate reductases linked with different thiol nucleophiles
| ArsCsa | Nucleophiles | Km | kcat | kcat/Km | H | References |
|---|---|---|---|---|---|---|
| mm | min–1 | m–1 s–1 | ||||
| Cg_ArsC1 | MSH/Mrx | 142 ± 19 | 32 ± 2.3 | 3.8 | 1 | This work |
| Cg_ArsC2 | MSH/Mrx | 82 ± 13 | 17 ± 1.2 | 3.4 | 1 | This work |
| Sc_Acr2p | GSH/Grx | 35 | 6 | 2.8 | 2.7 | Mukhopadhyay et al. (29) |
| Ec_ArsC R773 | GSH/Grx | 15 | 32 | 35 | 1 | Gladysheva et al. (15) |
| Os_Acr2 | GSH/Grx | 12 | 20 | 27 | 1 | Duan et al. (55) |
| Lm_Acr2 | GSH/Grx | 10 | 5.5 × 10–3 | 10–2 | 1 | Zhou et al. (18) |
| Sa_ArsC pI258 | Trx | 68 × 10–3 | 215 | 5.2 × 104 | 1 | Messens et al. (14) |
a
Cg, C. glutamicum; Sc, S. cerevisiae; Ec, E. coli; Os, Oryza sativa; Lm, L. major; Sa, S. aureus