TABLE 1.

Oligonucleotide sequences

Ø in the nucleotide sequence represents a THF residue, and B in the nucleotide sequence represents a biotin-conjugated strand.

Primer	Length in nt	Sequence
Upstream
U1	20	5′-CGACCGTGCCAGCCTAAAAC-3′
U2	19	5′-CGACCGTGCCAGCCTAAAA-3′
U3	26	5′-CGCCAGGGTTTTCCCAGTCACGACCA-3′
U4	25	5′-CGCCAGGGTTTTCCCAGTCACGACC-3′
U5	24	5′-CGCCAGGGTTTTCCCAGTCACGAC-3′
U6	22	5′-CGCCAGGGTTTTCCCAGTCACG-3′
U7	20	5′-CGCCAGGGTTTTCCCAGTCA-3′
Downstream
D1	55	5′-CGACCGTGCCAGCCTAAAAØACTTGCCCGTGCCACCATCCCGACGCCACCTCCTG-3′
D2	35	5′-ØCTTGCCCGTGCCACCATCCCGACGCCACCTCCTG-3′
D3	36	5′-ØACTTGCCCGTGCCACCATCCCGACGCCACCTCCTG-3′
D4	28	5′-GCCGTCGTTTTACAACGACGTGACTGGG-3′
D5	35	5′-ACTTGCCCGTGCCACCATCCCGACGCCACCTCCTG-3′
D6	53	5′-BTTCACGCCTGTTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG-3′
Template
T1	49	3′-GCGGTCCCAAAAGGGTCAGTGCTGGGCAAAATGTTGCTGCACTGACCCG-5′
T2	56	3′-GCTGGCACGGTCGGATTTTGTGAACGGGCACGGTGGTAGGGCTGCGGTGGAGGACG-5′
T3	53	3′-GCTGGCACGGTCGGATTTTGACGGGCACGGTGGTAGGGCTGCGGTGGAGGACG-5′