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Relative binding constants
| Substrate | Kda |
|---|---|
| nM | |
| 5-nt gap-flap | 0.36 ± 0.04 |
| 3-nt gap-flap | 0.41 ± 0.19 |
| 1-nt gap-flap | 0.55 ± 0.9 |
| Nick flap | 0.60 ± 0.12 |
| Double flap | 0.70 ± 0.21 |
Apparent dissociation constants measured by electrophoretic mobility gel shift assay. Results are the average of two independent experiments.
