Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 May 29;284(22):15173–15183. doi: 10.1074/jbc.M900322200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — The artificial chromophore precursor, 9-cis-retinyl acetate, preserves retinal structure and function in Lrat^-/-Rdh8^-/-Abca4^-/- mice. A, retinal histology of 6-week-old WT, Lrat^-/-, and Lrat^-/-Rdh8^-/-Abca4^-/- mice are shown (top). Cone photoreceptor degeneration is evident in the inferior retina of 6-week-old triple knock-out and Lrat^-/- mice. Cone photoreceptors and photoreceptor nuclei were stained with peanut agglutinin lectin (green) and DAPI (red), respectively. B, retinoid content of mouse eyes was determined by normal phase HPLC. Lrat^-/-Rdh8^-/-Abca4^-/- mice at the age of 6 weeks were gavaged once with 2 mg of 9-cis-retinyl acetate and kept in the dark for 3 days before analyses. Peaks corresponding to 9-cis-retinal were detected in gavaged Lrat^-/-Rdh8^-/-Abca4^-/- mice. atRE, all-trans-retinylester; 11cRAL, 11-cis-retinal; atRAL, all-trans-retinal; atROL, all-trans-retinol; 9cRAL, 9-cis-retinal; 9cRAc, 9-cis-retinyl acetate. C, scotopic ERGs were recorded from 6-week-old Lrat^-/- Rdh8^-/-Abca4^-/- mice. Representative ERG traces before (right) and after gavage with 2 mg of 9-cis-retinyl acetate (left) are presented. Retinal function was significantly preserved by pretreatment with 9-cis-retinyl acetate.