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. 2009 May 29;284(22):15173–15183. doi: 10.1074/jbc.M900322200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Regeneration of isorhodopsin with 9-cis-retinyl acetate in Lrat^-/-Rdh8^-/-Abca4^-/- mice. A, 6-week-old Lrat^-/-Rdh8^-/-Abca4^-/- mice were gavaged once with 2 mg of 9-cis-retinyl acetate (9cRAc) and kept in the dark for 3 days. Then mice were exposed for 12 or 24 min to bright light (intensity 500 cd·m^-2), and amounts of 9-cis-retinal and all-trans-retinal in the eyes were determined by normal phase HPLC. Means ± S.E. are presented (n > 3). B, HPLC was used to quantify amounts of A2E in the eyes of 6-week-old Lrat^-/-Rdh8^-/-Abca4^-/- mice after the indicated numbers of cycles (left and center), each consisting of a 2-mg 9-cis-retinyl acetate gavage followed by 3 days of dark adaptation and then a 24-min exposure to bright light (intensity 500 cd·m^-2). A2E levels in untreated control mice (double knockouts; 6-week-old Rdh8^-/-Abca4^-/- mice) are shown on the right. Means ± S.E. are presented (n > 3).