TABLE 1.
Oxidative properties of mitochondria isolated from the hearts of Sprague Dawley rats
Assays were carried out as described under “Experimental Procedures.” Glutamate was used as substrate. RCR, respiratory control ratio (state 3/state 4); ADP/O, ADP to atomic oxygen phosphorylation ratio (54); DNP, dinitrophenol. Respiratory rates are expressed as nanoatoms of oxygen/min/mg of mitochondrial protein. n = 3 (mean ± S.E. of measurement).
| State 3 | State 4 | RCR | ADP/O | DNP | |
|---|---|---|---|---|---|
| All-trans-retinal | |||||
| 0 μm | 225.0 ± 31.6 | 28.5 ± 4.0 | 8.2 ± 1.5 | 3.1 ± 0.1 | 284.9 ± 49.8 |
| 12 μm | 196.3 ± 21.0 | 29.0 ± 3.6 | 7.1 ± 1.5 | 3.5 ± 0.5 | 256.4 ± 32.5 |
| 25 μm | 187.3 ± 19.4 | 33.0 ± 3.0 | 5.7 ± 0.2 | 3.4 ± 0.1 | 222.0 ± 23.4 |
| 50 μm | 178.3 ± 14.2a | 36.3 ± 4.9 | 5.5 ± 1.4 | 3.1 ± 0.02 | 201.0 ± 26.7a |
| 75 μm | 153.0 ± 13.4a | 87.0 ± 3.0a | 1.8 ± 0.1a | 2.9 ± 0.2 | 30.0 ± 6.0a |
| 100 μm | 45.0 ± 10.4a | 45.0 ± 10.4 | 1 ± 0a | 0 ± 0a | 5.5 ± 0.1a |
| A2E | |||||
| 120 μm | 228.0 ± 21.6 | 40.7 ± 4.3 | 5.7 ± 0.5 | 3.1 ± 0.1 | 290.9 ± 30.3 |
| 240 μm | 225.0 ± 27.5 | 37.7 ± 3.8 | 6.0 ± 0.1 | 2.9 ± 0.1 | 296.9 ± 31.5 |
| 600 μm | 213.0 ± 19.7 | 39.0 ± 3.0 | 5.5 ± 0.1 | 3.0 ± 0.1 | 272.9 ± 21.5 |
a
p < 0.05.