FIGURE 6.

Screen for small molecules that induce MxA and inhibit motility. A, the Diversity Set of 1990 pharmacophores from the NCI Developmental Therapeutics Program was tested in a high-throughput screen using an MxA promoter-luciferase reporter assay. PC-3M cells were incubated for 24 h with 10 μm compound. Interferon-α was used as a positive control. Results from three small molecules that induced a >2.2-fold increase in activity are shown (two independent experiments in triplicate). B, effect of the three small molecule hits on PC-3M motility (two independent experiments in duplicate). Motility of PC-3M cells, with and without 24-h exposure to 1000 international units/ml IFN-α or the small molecule hits. Bars represent the percentage of control PC-3M mobility. Motility was assayed as described under “Experimental Procedures.” C, immunocytochemistry with anti-MxA antibody confirmed induction of MxA protein by the three compounds detected in the high-throughput assay for MxA promoter activation.