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. 2009 May 29;284(22):15277–15288. doi: 10.1074/jbc.M900365200

FIGURE 4.

FIGURE 4.

Effects of ERs on endogenous gene expression. A, summary of E2-ER-responsive genes identified with microarrays. MDA-MB-231 cells were infected with recombinant adenoviruses in the absence of E2 for 48 h. Cells were then treated with 10-9 m E2 for 6 h. Total RNA was subjected to microarrays. Results are the mean of six independent determinations. B, verification by qPCR of a subset of the genes identified by microarrays. MDA-MB-231 cells were infected with recombinant adenoviruses for 48 h. Cells were then treated without (data not shown) or with of 10-9 m E2 for 6 and 24 h. Total RNA from infected cells was processed for and subjected to qPCR. ERα in response to E2 augmented the expression of the C3, CDKNA1, and TBXR2R genes, whereas E2-ERα repressed the CD44, PLAUR, and WT1 gene expressions. The transcription of the FST gene was regulated only by E2-ERαEBD. On the other hand, both E2-ERα and E2-ERαEBD altered the ANKRD1, CRISPLD2, CTGF, HAS2, MMP1, MMP3, and SERPINB2 gene expressions. Results, which are the means ± S.E. of three independent determinations, depict relative change in mRNA levels compared with those observed in cells infected with the parent Ad5 in the absence of E2, which is set to 1. Asterisk indicates significant change.