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. 2009 May 29;284(22):15277–15288. doi: 10.1074/jbc.M900365200

FIGURE 7.

FIGURE 7.

Effects of ERs on death and motility of MDA-MB-231 cells. A and B, infected MDA-MB-231 cells in the absence or presence of 10-9 m E2 were subjected to an annexin V assay using FACS (A) or a TUNEL assay at 96 h post-infection(B). Results, which are the mean ± S.E. of three independent experiments, are summarized as the relative change in the number cells bound to annexin V or as the number cells that incorporated fluorescein isothiocyanate-conjugated dUTP into the fragmented DNA (TUNEL) obtained in comparison with the cells infected with the parent Ad5 in the absence of ligand, which is set to 1. C, wound healing assay. Infected cells were incubated in the absence or presence of 10-9 m E2 for 48 h to allow cells to reach near 100% confluence. A wound was then created, and images were captured at 0 h and at every 24 h thereafter. Results, which are the mean ± S.E. of three independent experiments performed in duplicate, are summarized as the wound closure at 96 h relative to 0 h. D, invasion assay. Cells were infected with recombinant adenoviruses in the absence or presence of 10-9 m E2 for 48 h. Cells were collected and counted. The same number of cells from each experimental group was then seeded on the upper section of the invasion chamber. After 24 h of incubation, cells on the bottom of the chamber membrane as an indication of invasiveness were stained and counted. Results, which represent the mean ± S.E. of three independent experiments performed in duplicate, are relative changes compared with cells infected with the parent adenovirus in the absence of E2, which is set to 1. Asterisk indicates significant change.