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. 2009 May 29;284(22):15299–15307. doi: 10.1074/jbc.M807285200

FIGURE 5.

FIGURE 5.

LRP6 BP12 and BP34 are functional units displaying different preferences for the LRP6 ligands: Wnt3a, Dkk1, and Mesd. Solid phase binding assays in which A, recombinant Wnt3a, B, recombinant Dkk1, and C, recombinant Mesd were coated onto the 96-well plates, followed by the incubation of control, LRP6 BP1234, BP12, or BP34 CM. CM, conditioned media. The amount of secreted BP1234, BP12, and BP34 in CM were previously quantified by in comparison to Fc-LRP6 pure protein (R&D) with known concentrations in Western blot analysis, and the same amount of BPs was applied. Values are the average of triple determinations with the S.D. indicated by error bars. Note that Wnt3a and Mesd display a preference for BP12, whereas Dkk1 binds better to BP34. *, p < 0.05; **, p < 0.01 compared with control CM.