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LRP6 BP1234, BP12, and BP34 directly interact with LRP6 ligands Wnt3a, Dkk1, and Mesd. BP CM was incubated with recombinant Wnt3a (A), recombinant Dkk1 (B), and recombinant Mesd (C), immunoprecipitated with anti-HA antibody or normal IgG (data not shown), and then probed with anti-Wnt3a, anti-Dkk1, or anti-Mesd antibodies to detect the indicated proteins. WB was analyzed via 7.5% (A) or 15% (B and C) SDS gels under reducing conditions. IP, immunoprecipitation; WB, Western blot.
