FIGURE 7.

LRP6 BP12 and BP34 CM inhibit Wnt signaling. HEK293T/STF cells were incubated with L cells CM, Wnt3a CM, or Wnt3a CM along with control or LRP6 BP CM at 37 °C for 16 h. BP12 and BP34 CM contain ∼30 μg of BP12 or BP34 protein, respectively, quantified by Western blot. Control CM containing equal amounts of total protein as BP CM was added to cells as negative control. Values are the average of triple determinations with the S.D. indicated by error bars. Note that both LRP6 BP12 and BP34 CM partially inhibit Wnt3a-induced Wnt signaling. ^**, p < 0.01 compared with control medium.