Mesd antagonizes Wnt3a binding to LRP6 BP1234, BP12, and BP34.
Recombinant Mesd (1 μm) is able to compete for rWnt3a (1
μg/ml) binding to BPs in co-immunoprecipitation (Co-IP) (A) and
solid phase binding assays (C). B, quantification of results
from Co-IP. D, recombinant Mesd (1 μm) is able to
compete for Myc-Dkk1 binding to BPs in Co-IP. IP,
immunoprecipitation; WB, Western blot.