FIGURE 13.

PLD2 siRNA attenuates hyperoxia-induced redistribution and tyrosine phosphorylation of cortactin. A, HPAECs were transfected with scrambled or PLD2 siRNA prior to exposure to either normoxia or hyperoxia (3 h), probed with anti-cortactin antibody, and examined by immunofluorescence microscopy using a ×60 oil objective. Exposure to hyperoxia resulted in redistribution of cortactin to cell periphery, which was attenuated by PLD2 siRNA. Shown is a representative immunofluorescence micrograph from several independent experiments. B, HPAECs were transfected with scrambled or PLD2 siRNA prior to exposure to either normoxia or hyperoxia. Cell lysates were subjected to SDS-PAGE and Western blotted with anti-cortactin, -phospho-cortactin, -actin, and -PLD2 antibodies. PLD2 siRNA attenuated hyperoxia-induced phosphorylation of cortactin. Shown is a representative blot from three independent experiments.