Table 1.
Cytokine, Chemokine and Nitrite in the Culture Supernatant Fluids of Murine MTB-Infected Bone-Marrow-Derived Macrophages Stimulated with the MALP-2 Derivative or LPS
| Stimuli | Days | MOI | TNF-α | IL-6 | IL-10 | MIP-1β | NO2 |
|---|---|---|---|---|---|---|---|
| pg/ml | pg/ml | pg/ml | pg/ml | µMol/L | |||
| Untreated | 2 | 1:1 | 0 | 188±62 | 92±2 | 1068±38 | 4.34±0.9 |
| 8 | 1:0.1 | 0 | 207±52 | 304±69 | 8078±148 | n.d. | |
| MALP-2 | 2 | 1:1 | 25±10 | 824±23* | 129±10 | 970±40 | 13.05±0.75* |
| 5 ng/ml | 8 | 1:0.1 | 0 | 211±53 | 260±15 | 7000±75* | n.d. |
| MALP-2 | 2 | 1:1 | 20±5 | 1243±7* | 102±6 | 1112±25 | 19.25±0.35** |
| 50 ng/ml | 8 | 1:0.1 | 0 | 301±44 | 418±90 | 6587±70* | n.d. |
| MALP-2 | 2 | 1:1 | 70±6* | 1470±64* | 126±6 | 1537+19* | 50.65±0.95** |
| 500 ng/ml | 8 | 1:0.1 | 10±5 | 538±151* | 361±89 | 5821±115* | n.d |
| LPS | 2 | 1:1 | 1879±39**,## | 2138±88**,# | 109±12 | 6846±225**,## | 142.4±1.8**,## |
| 500 ng/ml | 8 | 1:0.1 | 336±52**,## | 1284±69**,# | 504±91 | 6709±217* | n.d. |
a)Murine macrophages were infected with MTB at MOI 1:1 for 2 days or at MOI 1:0.1 for 8 days and cultured in the presence of LPS or the MALP-2 derivative at the indicated concentration. Culture supernatants harvested at the indicated time points were used for IL-6, IL-10, TNF-α , MIP-1β detection by specific quantitative sandwich ELISA Kits. The results are presented as mean.
± SEM in pg/ml. Pooled data of 3 independent experiments are shown. Nitrite was detected at 2 days with a specific colorimetric kit. The results are presented as mean ± SEM in µ Mol/L. Pooled data of 3 independent experiments are shown.
p<0.05 and
p<0.01, level of statistical significance between cytokine or nitrite release in stimulated versus untreated macrophages.
p<0.05 and
p<0.01, level of statistical significance between cytokine or nitrite release in LPS-treated versus MALP-2 derivative-stimulated macrophages.