Figure 1.
Properties of the monoclonal antibody against IGHMBP2. (A) Total cell lysates from mouse MN1 cells or human 293T cells were resolved on 4–12% NuPAGE gel, blotted and probed with 11-24 mAb. Molecular mass markers (in kilodaltons) are shown on the right. (B) Indirect immunofluorescence of IGHMBP2 on NT2 cell line using 11-24 mAb analyzed by epifluorescence microscopy. (C) FLAG-IGHMBP2 was in vitro-translated in the presence of 35S-methionine, and radiolabeled FLAG-IGHMBP2 was immunoprecipitated with non-immune mouse serum (NMS), immune serum from a mouse immunized with recombinant IGHMBP2 (IS-2), the 11-24 mAb, or anti-FLAG (M2) mAb. Immunoprecipitated proteins were resolved on 4–12% NuPAGE gel and detected by autoradiography. Total (T) contains 20% of input used for IPs.